Product Name

Respiratory V (lung infection) - Cat Nucleic Acid Test Kit (Dry) (FHV, FCV, Mycoplasma, Chlamydia, B. bronchiseptica)

Features:

• Product Name: PCR Reagent
• Storage Condition: 2-8℃ Away From Light
• Template Dna: Not Included
• Catalog Number: K1081
• Quality Control: Endonuclease And Exonuclease Activity Tested
• Pcr Time: 45min
• PCR reaction mixture
• PCR reaction cocktail
• Polymerase Chain Reaction Reagent

Technical Parameters:

Testing Principle

Testing Principle】 The test kit uses nucleic acid extraction reagents to extract the nucleic acid (DNA/RNA) from the sample. Under the action of a high-efficiency reverse transcriptase, cDNA complementary to the RNA template is synthesized in a one-step reaction using RNA as the template. Under the action of Taq enzyme, the copy number of the specific target fragment is amplified through cycles of high-temperature denaturation, annealing at a moderate temperature, and extension using DNA as the template. The fluorescence-labeled specific probe hybridizes with the amplified target fragment, and the 5'→3' exonuclease activity of Taq polymerase separates the reporting group and quencher group of the fluorescence probe, emitting a specific f luorescence signal. The specific fluorescence signal is detected using a f luorescence PCR instrument, and the result is determined based on the Ct value of the sample and the formation of the amplification curve.

Storage conditions and shelf life

1. Shelf life: 18 months.

2. Production date and expiration date are on the package

Compatible Instruments

This test kit is compatible with real-time quantitative f luorescence PCR instrument.