Sheep Insulin-Like Growth Factor-I,Somatomedin,IGF-I ELISA kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IGF-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IGF-1 and HRP conjugated IGF-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IGF-1. You can calculate the concentration of IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF-1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF-1 were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Assay range

0.3ng/ml -20 ng/ml

Sensitivity:

0.05ng/ml

Notes:

• Store the kit at 4°C upon receipt.The kit should be equilibrated to room temperature before the assay. Remove any unneeded strips from Bovine IGF-1 Antibody-Coated plate, reseal them in zip-lock foil and keep at 4°C.
• Precipitates may appear in concentrated washing buffer. Please heat the buffer to dissolve all the precipitates, which will not affect the results.
• Accurate pipette should be used to avoid experimental error. Samples should be added to the Microplate in less than 5 minutes. If a large number of samples are included, multiple channel pipette is recommended.
• Standard curve should be included in every assay. Replicate wells are recommended. If the OD value of the sample is greater than the first well of standards, please dilute the sample (n times) before test. When calculating the origina IGF-1 concentration, please multiply the total dilution factor (XnX5).
• In order to avoid cross-contamination, Microplate sealers are for one-time use only.
• Please keep Substrate away from light.
• All the operation should be accordance with the manufacturer's instructions strictly. The results determined by the Microtiter Plate Reader.
• All the samples, washing buffer and wastes should be treated as infectious agents.
• Reagents from different lots should not be mixed.

Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro virus, Parvo virus test kits, our products include rapid test cassette and Elisa kits.

All our products have been registered in National Medical Products Administration of China, in year 2020 we get Certificate of ISO 13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from SGS. More importantly, we have awarded the registration certificate from National Medical Products Administration for Diagnostic Kit for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during the outbreak in China, and also we developed COVID-19 Antigen test ; Neutralizing Antibody Test, these products selling well all overthe world